Zhang Yifang
May 9, 2022  

CURRICULUM VITAE

 

PERSONAL INFORMATION                 

Name:     Yi-fang Zhang   Ph.D.  
Dept. of Veterinary Medicine, Yunnan Agricultural University

E-mail:    zyfkm@yahoo.com.cn

Address:   Dept. of Veterinary Medicine ,Yunnan Agriculture University
       Kunnming 650201,  P.R.China,
          

Date of Birth:   Nev.12,1963

Sex:           Male

Family Status:  Married and one son

Nationality:    Chinese

Condition of health: Excellent

 

Teaching Course

Veterinary Microbiology and Immunology for undergraduate students.

Veterinary Bacterial, Veterinary Virology, Veterinary Immunology for graduate students

Membership:

Member & Director of Chinese Society of Animal Infectious Disease.

Member & Director of Society of Immunology in Yunnan Province.

Member & Director of Society of Microbilogy in Yunnan Province.

 

EDUCATION  EXPERIENCE

2007~2008: Visiting Scholar

           Molecular Lab., Department of Microbiology,

          University College Cork, Ireland

1998~2001: Ph.D.             

          Major: Preventive Veterinary Medicine

          Changcun Veterinary University (The Quartermaster University of PLA)

1985~1988: M.S 

           Major: Veterinary microbiology and immunology

           Gansu Agricultural University

1978~1982: B.S

           Major: Veterinary Medicine

           Yunnan Agricultural University

 

RESEARCH  EXPERIENCE

2001-present: faculty (professor)

Laboratory of Veterinary microbiology and immunology,

Yunnan Agricultural University, P.R. China.

Research scientist

Center of Monoclone antibody engineering technique ,

Yunnan University, P.R. China.

1988-1998: faculty (Assistant Professor &Associate Professor)

Department of Veterinary Medicine ,

Yunnan Agricultural University, P.R. China.

1982—1985: District Veterinarian

Xianwei district, Yunnan province, P.R. China.

 

RESEARCH  SUBJECT

A study on epidemiology and etiology of Major disease of animal and poultry diseases. National Key Research and Development Program (Project Number 2017YFD0500105) 2017-2020

Molecular mechanisms of dairy cows tuberculosis susceptibility and Polymorphisms of Card 15/SLC11A1/IFN-γ gene.National Nature Science Fund of China(Project Number 31260598), 2013-2016

A study on prevention and diagnosis of Hog cholera virus(Swine fever virus) & Porcine reproductive and respiratory virus. Project of Yunnan province science fund (Project Number  ), 2010-2012

Clone and expression of gene of Lactobacillus species. Project of Ireland’ natural science fund (Project Number),2007.12--2008.12.

A Study on detective technique of bovine tuberculosis. Project of Yunnnan province’ natural science fund (Project Number2002NG03), 2002-2005

A Study on gene engineering antigen of Streptococcus mutans. Project of Yunnnan province’ natural science fund (Project Number ),2002-2004

A Resaerch and demonstration of pathogeny diagnosis and rapid detective technique of avian influenza virus. Project of Yunnnan province’ natural science fund (Project Number2004NG01),2004-2005

A Study on detective strip of antigen of Hog cholera virus (Swine fever virus). Project of Yunnnan province’ natural science fund (Project Number2002YP06),2002-2005

A Study on epidemiology and preventive technique of bluetongue virus. Project of Chinese Agricultural Ministry (Project Number 9605020103), the cooperative Research Project of Chinese-Australian(Project Number ACIAR 9301), 1998-2001

A study on epidemiology and Immunology of Hog cholera virus(Swine fever virus). Project of national natural science fund (Project Number 39893290-1), 1996-2000

A study on resource investigation and using of Lactobacillus species. Project of Yunnnan province’ natural science fund (Project Number),1992-1995.

A study on biological characteristic of polysaccharide from Spirulina: Project of Yunnnan province’ natural science fund, 1992-1995.

A study on immunological diagnosis technique of Pasteuralla multocida. Project of Guansu Province’ natural science fund(Project Number), 1985-1988

 

PUBLICATIONS

[1] Biao He, Wenjie Gong, Xiaomin Yan, Zihan Zhao, Ling’en Yang, Zhizhou Tan, Lin Xu, Aiwei Zhu, Jianing Zhang, Jihong Rao, Xinglong Yu, Jianfeng Jiang, Zongji Lu, Yifang Zhang, Jianmin Wu, Yong Li, Yuxiang Shi, Qing Jiang, Xiwen Chen, Changchun Tu; Viral Metagenome-Based Precision Surveillance of Pig Population at Large Scale Reveals Viromic Signatures of Sample Types and Influence of Farming Management on Pig Virome, mSystems, 2021,6(3):1-18 mSystems, e00420-21,page 1-18msystems.asm.org

[2] Jun Chai, Qinglu Wang, Bo Qin, Shengkui Wang, Youtao Wang,Muhammad Shahid,Kai LiuYifang Zhang,Weijie Qu;Association of NOS2A gene polymorphisms with susceptibility to bovine tuberculosis in Chinese Holstein cattle,Plos One,June 17,2021 |https://doi.org/10.1371/journal.pone.0253339 2021

[3] NianciLv,LiZhu, Wengui Li,Zhilan Li, Qisheng Qian, Tianyu Zhang, Lu Liu, Jinmei Hong, Xiaolin Zheng, Yuan Wang, Yifang Zhang*,Jun Chai*;Molecular epidemiology and genetic variation analyses of porcine circovirus type 2 isolated from Yunnan Province in China from 2016-2019, BMC Veterinary Research (2020) 16:96

[4] Ting Ouyang, Guyu Niu ,Yifang Zhang , Xiaohua Liu,Xinwei Zhang,Shiqi Zhang,Yulu Geng,Daxin Pang,Hongsheng Ouyang and Linzhu Ren; Porcine HMGCR Inhibits Porcine Circovirus Type 2 Infection by Directly Interacting with the Viral Proteins, Viruses, 2019, 11, 544; doi:10.3390/v11060544 

[5] Xiaolin Zheng ; Bo Qin ; Qinglu Wang L; Youtao Wang ; Qishun Liao ; Jinling Zheng ; Guodong Dong ; Yifang Zhang; Xianwei Shi; Jun Chai*; Association of BoLA-DRB3.2 gene polymorphism with Tuberculosis susceptibility of dairy cattle in Chinese Holstein cattle; Journal of Tuberculosis; 2019;2(1):1010.

[6] Kaihua Liu, Bin Zhang, Zhaochun Teng, Youtao Wang , Guodong Dong, Cong Xu, Bo Qin, Chunlian SongJun Chai, Yang Li.Xianwei Shi, Xianghua Shu ;Yifang Zhang*;Association between SLC11A1 (NRAMP1) gene polymorphisms and susceptibility to tuberculosis in dairy cattle,Tuberculosis,103201710-15

[7] Nana ZHANG, Qingxia LU, Man TENG; Yanyan YANG; Yifang ZHANG*; Gaiping Zhang*; Method and Progress of the Research on Virus Affinity Peptide; Agriculture Basic Science and Technology, 2016,17(12):2713-2716

[8] Youtao Wang, Shengkui Wang, Tong Liu, Wenji Tu, Wengui Li,Guodong Dong, Cong Xu , Bo Qin, Kaihua Liu , Jie Yang, Jun Chai, Xianwei Shi,Yifang Zhang*;CARD15 Gene Polymorphisms Are Associated with Tuberculosis Susceptibility in Chinese Holstein Cows;PLOS ONE/journal.pone.0135085,August.5.2015

[9] Xiangru Wen, Kai Wan, Ziming Zhao,Yifang Zhang, Tingting Sun, Fang Zhang, Jian Wu,Yanyan Fu, Yang Du, Lei Zhang,Ying Sun, YongHai Liu, Kai Ma Hongzhi Liu*, Yuanjian Song;  Brain-Targeted Delivery of Trans-Activating Transcriptor-Conjugated Magnetic PLGA/Lipid Nanoparticles; PLOS ONE,9(9): e106652,

[10] WANG Qing-lu, ZHANG Shu-guang, LI Fu-xiang, BI Bao-liang, CHAI Jun, WANG Sheng-kui and ZHANG Yi-fang*;Isolation and open reading frame 5 gene analysis of porcine reproductive and respiratory syndrome virus in Yunnan Province, China, African Journal of Biotechnology, 2012,11(90):15728-15732,

[11] Yifang Zhang Xiaogang DuWengui Li.Jianping LiuJianbo ZhuNianzu Zhang;Genetic Diversity of the S10 RNA Segment of field and vaccine strains of bluetongue virus from the P. R.China; Archives of Virology, 2010, 155: 281-286

[12] Liu X.C., Zhang Y.F. at el; Diagnosis and prevention and treatment in mixed infection with Coccidia and E.Coli in Rabbits, Progress in Veterinary Medicine; 200930(4)123—125
LI Jin-Tao
BI Bao-Liang, XC LiuLI Yan-BingY GuiLI Zuo-ShengYF Zhang; The Establishment and Preliminary Application of Detection Method of Mixed Infection both Classical Swine Fever and Porcine Circovirus Type 2, Chinese Journal of animal husbandry Veterinary Medicine, 2009,36(4):69-72

[13] Zhu W.H., Zhang Y.F.Cloning and Expression of E2 Gene of Classic Swine Fever Virus; Henan Agricultural Science 2009,9:180-183

[14] GUI Y.LI Q.Li Y.BLi J.TXiao J.Tong L.Shu X.T.Liu X.C.Li Z.S.Zhang Y.F.The Applied Research on the RT-PCR Nucleic Acid Detection and ELISA Antigen Detection Kit of CSFV; Journal of animal husbandry Veterinary Medicine,2009 ,36(7):184-186

[15] Cao X.P., Zhang Y.F. at el; Detection of Classical Swine fever virus by using sandwich ELISA and immune fluonesenic antibody technique. Chinese Journal of Veterinary Medicine,200844(1):66-67

[16] Liao hongfang; Zhang Y..F.; Diagnosis of Sheep pox and Goatpox Virus from Yunnan by using PCR and Electric microscope technique; Chinese Journal of Veterinary Medicine, 2008,44(1):30-32

[17] Cao xingping; ZhangY.F.; at el; Investigation of serum epidemiology of porcine circovirus type2 from Yunnan  Cuxiong; Chinese Journal of Veterinary Medicine,2008,44(2):38-40,

Wu X., Wang J., Zhang Y.F.;Development and application of One Step RT-PCR Assay for detecting Classical Swine fever virus;Chinese Journal of animal husbandry and Veterinary Medicine,2008,35(2):64-66

[18] Zhao H.Z., Wang S.K., Zhang Y.F.; Isolation,identification and drug resistant test of Swine streptococcus; Hubei Journal of animal husbandry and veterinary science; 2008(5):7-8,10

[19] Zhao H.Z.,Wang S.K.,Zhang Y.F.; Serological detection and pathogenic analysis of Swine streptococcus from Kunming; Animal Husbandry & Veterinary Medicine 2008,40(5):60-61

[20] Li Q., Zhang Y.F. at el; RT-PCR Diagnosis and treatment in mixed infection with Classical Swine fever virus and Porcine reproductive and respiratory virus; Chinese Journal of animal husbandry and Veterinary Medicine;2007,34(12):95-96

[21] Cao X.P., Zhang Y.F.;Study on seroepidemiological survey of Porcine Pseudorabies,

Chinese Journal of animal quarantine, 2006231139-41

[22] Gue C.X, Zhang Y.F.;Study and applied of Lactobacillus serve as recipient bacteria,China Biotechnology,2006,26(Suppl):212-216

[23] Jiang C.Y, Zhang Y.F.;Study and applied of T4 phage surface display techniqueChinese Journal of Zoonosis, 2005,21(11):1011-1013

[24] Luojiaqin,Zhang Y.F.;Study on Cloning and Expression of ESAT-6 gene Bovine Tuberculosis. The sixth Chinese Symposium on human and animal infection ,September,2004,Hofei,China.(Abstract)

[25] Zhang Y.F, Du Xiao-gang, Malan;Study on Cloning and Expression of pac Gene of Streptococcus mutans;Bioresourrces and Biotechnology International Symposium, August 2004, Kunming,China

[26] Zhang Y.F ; Liu jianping; Zhu jianbo; Zhang nianzu; Phylogenetic analysis of S10 gene Segment of Bluetongue virus from the People’s Republic of China,Bioresourrces and Biotechnology Intermational Symposium,August 2004,Kunming,China

[27] Luojiaqin, Llifuxiang, Zhang Y.F.;Advancements on Intracellular Infection. Journal of Microbiology 2004,2,24(2):46-49

[28] Li fuxiang, Luo jiaqin, Zhang Y.F.; Biological characteristic and Application of Single-chain fragment variable antibody. Current immunology 2004,24(3):253—255

[29] Zhang Y.F., Zhangnianzu, Liujianping; Genetic Diversity Analysis of the S10 Gene of field and vaccine strains of Bluetongue virus. Acta Microbiologica Sinica 2003.43(6):698-705

[30] Zhang Y.F., Liu jianping, Zhu jianpo et.al. Genetics diversity of S10 gene segment between China field strain of serotype I and vaccine strain of bluetongue virus. China journal of veterinary science 2002,22(2):118—120

[31] Zhang Y.F., Liu jianping Zhu jianpo et.al. Cloning and sequence comparison of  L2 genes of  bluetongue virus from china. China journal of veterinary science 2002,22(11):536—538

[20] Zhang Y.F.,Situation and prospects of microorganism feeds. China feed 2001(3):12—13

[32] Zhang Y.F., Wang shengkui. Factors and improvements of the immune results of nucleate vaccine. Heilongjiang journal of animal science and veterinary medicine 2000,(3) :24-25

[33] Zhang Y.F., et.al. Isolation identification and ferment characteristic of lactobacillus from dairy fan product of dali in yunnan. China dairy cattle 2000(2)22--23

[34] Zhang Y.F., Luo fucheng, Liu xuchuan. Microbiological feed fermentation and processing technique of microbiological feed. Pratacultural science 2000,17(6):67--70

[35] Zhang Y.F.,Duan gang, Liu xuchuan. Inhibitory activity of polysacchride and polysacchride-protein extract from spirulina platensis on proliferation of cancer cells.

Marine sciences 2000,24(3):16--17

[36] Zhang Y.F., Wang sengkui,Zhang wenxing. The isolation and identification of loctobalillus spp.from broiler`s stomach. China poultry 2000,22(3):11--12

[37] Zhang Y.F., Liu xuchuan. Nutrients and medicinal value of spirulina platensis. Journal of yunnan agricultural university 2000,15(1):85--87

[38] Zhang Y.F., Zhang nianzhu. Detection of Blutongue virus by using nested-PCR according to BTV-17NS1 gene sequence. Progress in preventive veterinary medicine 2000,2(1):62--63

[39] Zhang Y.F., Xiang wenbing, Liu xuchuan. Isolation and identification of lactobacillus from crisp cream dairy dreg product of zhongdian in yunnan. Food science and technology 2000,(1):54—55

[40] Zhang Y.F., Liu xuchuan. Study on the anti-fatigue of polysaccharide and polysaccharide-protein from spirulina platensis. Marine science bulletin 1999,18(3)89--91

[41] Zhang Y.F., Xia fengyi, Liu xuchuan. Isolation identification and fermented characteristics of lactobacillus. China dairy industry 1999,27(3)22--24

[42] Zhang Y.F., Liu xuchuan, Li qihua. Study on characteristic and purification of spirulina phycocyanin. Journal of yunnan university 1999,21(3):230--232

[43] Liu xuchuan, Zhang Y.F.; Application of immunoenzyme-histochemical staining and rabbit-cross immunization for diagnosis of swine fever.Chinese journal of veterinary science and technology1999,29(6):27

[44] Zhang Y.F., Liu xichuan, Li zhixiang, et al. The investigation and study of lactobacillus resource from yunnan province. Journal of yunnan university 1998,20(2)124—127

[45] Zhang Y.F., Yang zhilei. The latest trends in research and product development of spirulina. Marine sciences 1998(5):68—70

[46] Zhangyifang, Liuxichuang, Lizhixiang, Liuyinwu. The Investigation and Study of Lactobcillus Resource. Journed of yunnan university 1998.20(2):124-127

[47] Zhang Y.F., Liu xichuan, Zhang wenxin, et al. The comparative study on the chromosomes of the saba pig and the dahe pig. Journal of yunnan agricultural university 1997,12(3)206—208

[48] Zhang Y.F., Li zhixiang, Hua cuncai, et. The Growth and Metabolic Kinetics of Lactic Acid Bacteria in Yohgurt. Food and Fermentation Industris1994,(2)41—43

[49] Zhang Y.F., Liu yinwu, Li zhixiang. Purification and Evaluation of Lactic Bacterial in Silage. China feed 1993,(10)24—25

[50] Liu Yinwu, Zhang Y.F., Zhao benching. The Isolation ,Identification of Lactobacillus spp.from Dairy Products. Microbiology 1993,20(4):203—206

[51] Zhang Y.F.,Study on Serological groups determination of pasteurella multocida by using immuno-fluorescence staining method.Chinese journal of animal and poultry infectious diseases1994(2):25--26

[52] Zhang Y.F.,Serological groups determination and detection of pasteurella multocida on smears from mice using immuno-enzyme histochemical method. Journal of yunnan agricultural university 1992,7(3):172--175

 

RESEARCH AND SKILL TRAINING

 I have had good training and research experience in the area:

Bacteriological experimental technique:

 Culture,isolation,identification,characterization and freeze-dry keeping of bacteria (bovine tuberculosis,Pasteuralla multocida,Streptococcus mutans,Lactobacillus Spp.,Spirulin plantensis).diagnosis and control of  animal bacterial disease.

Virulogical experimental technique: 

tissue or chicken embryo culture, isolation, identification and characterization of virus (Porcine circovirus, Swine fever virus, Porcine reproductive and respiratory virus, avian influenza virus, bluetongue virus). diagnosis and control of  animal virosis.

Immunological experimental technique:

direct and indirect Immunofluorescence antibody (DFA)assay, ezyme-linked immunoabesorbent assay(ELISA), direct immunoperoxidase staining assay, direct agglutination assay, indirect-blood agglutination assay, virus neutralisation assay, agar diffuse assay, gelose gelatin electrophoresis assay.

Preparation of labeling antibody(rabbit immunization, purification of IgG, immuno-enzyme label or fluorescent antiboidy label).

Preparation of monoclone antibody( (Preparation of antigen, mouse immunization, Preparation of spleen cell, Preparation of myeloma cells, cell fusion, cloning of hybrid cells)

Molecular biological experimental technique:

design of PCR and cloning primer, Extraction of DNA and RNA, PCR assay, RT-PCR assay, purification of PCR product and plasmid DNA, enzeme digestion and connect of DNA, construction and detection of recombinant (karoyotic & eukaryotic) plasmid, Preparation of E.coli sensitivity cells, transfection of plasmid.

expression and purification of recombinant protein, SDS-PAGE assay, west blotting assay, denature and renature of recombinant protein.

Sequence of nucleotde acids, phylogenetic analysis of nucleotde acids.